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1,10-Phenanthro-line-5,6-dione was prepared by a similar method reported in the literature [35]. All aqueous solutions were prepared with doubly distilled water.The Tris-HCl buffer was obtained from Tris (10 m M) and Na Cl (100 m M), and the p H value was adjusted to 7.2 with HCl (0.1 mol); this buffer was used for ultraviolet (UV) titration, fluorescence quenching thermal denaturation, circular dichroism (CD) spectra of CT-DNA.

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